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Order of lab manipulations (displaced topic)

Colonies Microbes Dye

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#1 Crocozz



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Posted 23 April 2020 - 05:54 AM



I'm sorry if my english isn't good enough but here is my problem (I displaced the topic from General Lab Techniques) :


I struggle with an exercise (on attach files) about the order of laboratory results. The goal of this exercise is to restore the correct order of manipulations.


As you know, the spreading of the Covid prevents our professor to give us a good course, and I'm a bit lost with all these lab' terminologies.


All the results are noted with a letter of alphabet : G to Q.


There are some notations on each picture :


*G = fixed smear with coloration of Wirtz Conklin

*I = bacillus

*K= Hektoen agar

*L= Rabbit plasma 


*N= Slanetz/Bartley agar

*O= Coccus

*P= +Lys / - Lys

*Q = Witness transplanting


To me, it seems that :


*bubles on O/I are the presence of catalase.

*Wirtz Conklin colored the endospore

*API gallery would be the last manipulation.


But I'm completely lost with the meaning of the results and the order of these results. 


Can you give me some help ?


Thank you very much !




 witness transplanting



Attached Files

#2 bob1


    Thelymitra pulchella

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Posted 23 April 2020 - 03:13 PM

Can you transcribe the actual question - it looks like you are trying to distinguish between cocci and bacilli with biochemical/staining tests?


If this is the case, think about how you would grow the organisms (which agar and what does that agar select for/against), then think about what result each organism will give with what test. These answers will be in the course notes somewhere - and certainly on the internet with some googling.

#3 Crocozz



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Posted 23 April 2020 - 10:56 PM

Thank you bob1 for your quick reply ! The question is all about the order of the photos. Each describes a step in the protocol but they must be restitute in a good order.

#4 Phil Geis

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Posted 23 June 2020 - 11:27 PM

Were you able to answer this?

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