I recently moved into a new lab that has a -80 C freezer, up untill now I used to freeze my cells in a standard -20 C freezer. the freezing protocol I'm using consists of a 1-step Glycerol:PBS 50:50 freeze.
I'm thinking about moving my stocks to the -80 C freezer, and would love to hear thoughts on why it would be better for them. Also, in case I'm deciding to move my currently -20 C frozen tubes into -80 C, is there any intermediate step I should be aware of?
My stock currently consists of:
E.Coli ATCC/ Listeria Mono ATCC/ Salmonella Thypi/ Candida Albicans ATCC/ Penicilum Rubens ATCC
And several more gram negative Bacteria.