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RT-PCR for cloning low expressed genes


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#1 mfpa

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Posted 30 September 2004 - 06:05 AM

For cloning low expressed genes by RT-PCR, what is better: RT-PCR with a big amount of tRNA (25ug tRNA/ 20ul reaction) or low amount of tRNA (1ug/20ul)???

#2 kawaka

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Posted 01 October 2004 - 05:11 PM

Why use tRNA in RT-PCR?




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