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cell free DNA (cfDNA)

elution buffer

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#1 SF_HK

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Posted 17 April 2020 - 12:03 AM

Hi,

 

Can anyone suggest a good elution buffer for cfDNA. I feel they are more easily degraded (-20deg celsius). We currently elute in deionised water.

 

Qiagen kits elute in AVE buffer, are they better?



#2 hobglobin

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Posted 17 April 2020 - 10:48 AM

Never worked with cfDNA, but with deionised water you have to be sure to have a fitting pH value, otherwise hydrolysis might occur. Buffers with EDTA have also the advantage to chelate  Mg2+ which are needed for enzyme activity (e.g. nucleases).

So an TE-buffer, pH 7 might be appropriate.

Anyway if you elute from a column I'd use the recommended buffer of the company or a clone of it.



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