julianne: been using sod acetate- isoprop precipiation with no problems all the while.
Turtle: my suggestion would be to ethanol precipitate,
for PCR product purification used for cloning, phenol/chloroform's classical method is ok. I think kits are unnecessary.
For cloning of PCR products, does phenol/chloroform extraction necessary? or can I just do a precipitation?
I have a basic question about DNA precipitation, sometimes isopropanol is used, sometimes ethanol is used, what is the reason for using one not the other, and what's the rule when to use one not the other?
Edited by bioforum, 29 September 2004 - 04:15 PM.