Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DNA extinction coefficients


  • Please log in to reply
1 reply to this topic

#1 netnus

netnus

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 31 posts
0
Neutral

Posted 29 September 2004 - 12:58 PM

Hi,everyone,

I just want to use spectrophotometer to measure the concentration of my DNA. But I don't know the DNA extinction coefficients.
Does anyone know how to calculate the extinction coefficients and any website I can calculate online?

Thanks!


netnus

Edited by netnus, 29 September 2004 - 12:58 PM.


#2 jadefalcon

jadefalcon

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 223 posts
1
Neutral

Posted 30 September 2004 - 05:50 AM

1 A260nm of ds DNA = 50g/ml H2O
1 A260nm of ss DNA = 33g/ml H2O

Pure DNA : A260nm/A280nm >= 1.8

1 A260nm of ss RNA = 40g/ml H2O

Pure RNA : A260nm/A280nm >= 2.0

mind that ODs should lie between 0.1 and 1.0 for correct measurements!

mike
--- He who finds typos may keep them! ---




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.