hi everyone,
i just want to know what they exactly mean by housekeeping genes. if a gene is labelled a "housekeeping gene" is it essential for the survival of the cell? thanks!
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caro
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Posted 04 November 2004 - 01:26 AM
Housekeeping genes are often genes involved in the maintenance of a cell, hence their name. In research they are often used as a control or used to be able to compare two different assays.
Normally you say (hope
) that they are expressed eaqually in all cells and tissues (they are not entirely, so you use more than one to overcome this, and from different pathways).
I have used it in Nothern and qRT-PCR, and you normalise against the housekeeping genes to get a (semi) quantitative measure.
Regards Caro
Normally you say (hope
) that they are expressed eaqually in all cells and tissues (they are not entirely, so you use more than one to overcome this, and from different pathways).I have used it in Nothern and qRT-PCR, and you normalise against the housekeeping genes to get a (semi) quantitative measure.
Regards Caro
