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housekeeping genes


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#1 uaue

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Posted 29 September 2004 - 08:25 AM

hi everyone,

i just want to know what they exactly mean by housekeeping genes. if a gene is labelled a "housekeeping gene" is it essential for the survival of the cell? thanks!

#2 caro

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Posted 04 November 2004 - 01:26 AM

Housekeeping genes are often genes involved in the maintenance of a cell, hence their name. In research they are often used as a control or used to be able to compare two different assays.
Normally you say (hope :unsure:) that they are expressed eaqually in all cells and tissues (they are not entirely, so you use more than one to overcome this, and from different pathways).
I have used it in Nothern and qRT-PCR, and you normalise against the housekeeping genes to get a (semi) quantitative measure.
Regards Caro




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