T- vector clone
Posted 27 September 2004 - 05:29 AM
I have got a 9.6kb with takara LA taq,I'm wanting to clone this insert using takara pMD-18T easy vector cloning system. I've tried many times,but the result is very undesirable!i am vrey confused.Is that the T-vector have not clone long DNA product?or others?If my DNA product have complex construcure?I do't know.when t have try it,i 've gel extracted it and eluted in 50ul water,ligation was done at 16oC overnight.then tranformation with complete JM109.
Do you spend your time tell me? what limit long PCR product clone to T-VECTOR?
i show my best whises to you !thanks very much!
Posted 04 October 2004 - 03:59 AM
thanks for all!
Posted 08 October 2004 - 12:15 AM
Edited by drjulianz, 08 October 2004 - 12:16 AM.
Posted 19 October 2004 - 12:01 AM