Posted 25 January 2001 - 10:00 PM
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Posted 02 February 2001 - 10:00 PM
Posted 25 October 2001 - 09:00 PM
Posted 30 March 2005 - 01:01 AM
I'd like to ask you if it is possible to overcome DNA contamination during RNA isolation. I use the TRIzol method and I always have DNA contamination.
Thanks in advance
Posted 30 March 2005 - 02:31 AM
for qiagen kit, does it allows isolation of siRNA too? i'm intersted on that point...
On the trizol manual, it's told that low ratio 260/280 may be due to a bad resuspension of rna. Try increase the amount of DEPC water of our prep. Will be better. One other possibility is to heat your rna 10' at 65° before make a OD measure.
To Pon103 :
try increase the amount of trizol you use for cell/tissue lysis. It will reduce contamination possibilities...