Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

question about stable transfection


  • Please log in to reply
1 reply to this topic

#1 mystery

mystery

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 26 September 2004 - 04:44 AM

:) I am doing stable transfection using G418 as the selection reagent. How often should I change the medium? I am changing it once every two days.

#2 kant0008

kant0008

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 90 posts
0
Neutral

Posted 26 September 2004 - 04:23 PM

Hi,
it depends on your cell growth also, but G418 is stable for about 4 days, so if your cells don't become overconfluent or start looking sick and like they need a "feed" once in 4 days should be ok




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.