I am doing stable transfection using G418 as the selection reagent. How often should I change the medium? I am changing it once every two days.
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mystery
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Posted 26 September 2004 - 04:44 AM
I am doing stable transfection using G418 as the selection reagent. How often should I change the medium? I am changing it once every two days.
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kant0008
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Posted 26 September 2004 - 04:23 PM
Hi,
it depends on your cell growth also, but G418 is stable for about 4 days, so if your cells don't become overconfluent or start looking sick and like they need a "feed" once in 4 days should be ok
it depends on your cell growth also, but G418 is stable for about 4 days, so if your cells don't become overconfluent or start looking sick and like they need a "feed" once in 4 days should be ok
