Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

LPS floursecent studies


  • Please log in to reply
1 reply to this topic

#1 Ger

Ger

    member

  • Members
  • Pip
  • 5 posts
0
Neutral

Posted 23 September 2004 - 05:14 PM

I would like to analyse the interactions of LPS with my cells, whether it is internalized etc.

Does anyone have a protocol?
Thank you.

#2 immune platelet

immune platelet

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 10 November 2004 - 08:02 AM

Sigma sells FITC-conjugated LPS. You can stain your cells in the presence of this LPS-FITC, but beware, you must have serum present in your staining system, which will provide the LPS binding protein (LBP). Stain for 30 minutes on ice for cell surface and 30-60 minutes at 37C for internalization. You may need to play with the LPS concentration and timing.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.