I'm working on a 40Kda His-tagged protein and I have too much contaminant protein after elution on Ni-Nta column.
I tried to wash with increased concentration of imidazole until my protein is eluted, but contaminant protein are eluted too. I tried gel filtration but contaminant protein still here. I think that there is too much protein interaction betwen my protein and contaminant.
How to reduce protein interaction?
I tried chaotropic salt (NaSCN) and increased salt concentration. with encouraging results but it's not enough.
Problem with contaminant protein after elution
No replies to this topic