I have been able to resolve the two ~500 bp bands in a size ladder. Instead of NaOH and boric acid, I use sodium tetraborate and boric acid, which is in the Biotechniques paper.
thabx alot for the SB buffer information. I used it but i m facing problems in resolution. I run 1kb marker on 0.8% agarose with 250volts for 30 min .
please help me if u have better results
The gels run fast, but there is no real buffering capacity, so you have to replace the buffer fairly regularly. I have tied recovering it and reusing it, but that's only good for a few runs. haven't tried 'spiking' the old buffer with fresh buffer occasionally, though, but it might work if you're really pressed for money.
How to cut cost in a molecular biolog lab?
Posted 05 February 2009 - 12:36 PM
Posted 05 February 2009 - 12:44 PM
Posted 25 December 2009 - 06:48 PM
In our lab we also pack our own tip boxes for non sterile purposes whilst buying barrier tips for molecular biology procedures. We even use glass pipettes for cell culture which we wash and bake rather than buying the plastic ones. We make and filter our own media (it's cheaper to buy the media powder form in bulk and make your own media than ordering indiviual bottles from the same company). As the above poster mentioned, company freebies are a good way of getting by when on a tight budget (one of the students in my lab pretty much did her phD experiments using freebies such as sample vectors, kits etc). Our lab doesn't use much kits, we often do things the old fashioned way, for example, we make up our own solutions for cloning. The most important advice the students of our lab are often told is to plan experiments well and always be aware of what one is doing so as to minimise errors and repeating of experiments, and hence wasting of reagents!
Posted 19 March 2011 - 05:51 PM
Here is another way of cutting lab cost - to reuse TAE buffer and even reuse the gel.
We reuse our polyacrylamide gels several times. I typically use mine 2-3 times a day, and make new every week. We put EtBr in the running buffer, take pix, and run the gel an hour or so before loading the next run. We use TBE, but I think I'll try bob's suggestion this week. Thanks bob!
Buying the pre-cast glass for sequencing gels can get expensive. Go to the local window glass shop and take one of the cracked originals with you. Ask to have them cut a replacement, which cost us about $15/plate. They can cut the ears, too; tell them to drill a pilot hole in each corner before making the cuts to avoid breaking the glass.
Rain-Ex (car window water repellant) is the same as Gel-Repel, and can be purchased much more cheaply. If you don't need to transfer the gels, then you can skip treating the glass.
A fish tank thermometer strip is an alternative to gel strips.
Use isopropyl alcohol instead of ethanol to precipitate DNA. You only need to add 2/3 isopropanol to the aqueous layer and put in the freezer for an hour before centrifugation. This means you can do extractions in 1.5 ml tube, so you only use 3 tubes to do a single CTAB-chloroform extraction. (CTAB/boil, chloroform, aqueous solution/precipitation and storage)
Posted 14 December 2011 - 09:34 AM
We used standard protocol from ABI (BigDye master mix), 4 ul of mix, 2 ul of sequencing buffer in 20 ul reaction.
I learned on the workshop that this could be done using only half in half a volume.
So I tested several variants, and ended with using 1 ul of mix, 1.5 ul of buffer in 10 ul volume (you need good tightly capped tubes though), which uses only 1/4 of original mix for the same reaction. There is no difference in the results and no changes in run program. It even cuts the cost of purification mix (XTerminator) by half due to the reduced volume, but purification can be made also by cheap ethanol method without any kit, just that the kit gives cleaner beginning reads.
I found a page about plasmid sequencing, that went really extreme in diluting the mix, but I don't thing I'd like to go that far by increasing the number of cycles or so.
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
I never trust anything that can't be doubted.
'Normal' is a dryer setting. - Elizabeth Moon
Posted 28 August 2012 - 06:46 PM
You should use new brand product.
as crazy as this sounds, my director told us to cut cost in our lab..
i am at my wits end on thinking how to do this?
we have already been very careful in our spending..
all this while we use as much 'non-kit' as possible even though its a pain you know where!!! kits are expensive in our place..
i know of labs where they actually recycle their tips.. but i think that's a big NO-NO...
my neurons are just snapping off thinking about this..
what i think we should do is fire the management and hire new lab staff.
this way we save cost (the directors and managements obscene salary) and increase productivity at the same time! (just kidding)
This is a new lab equipment company.
Some product is good.
And bandbeeper is very good.
Many type illuminator
E-cooler is cool.