ChIP Assay and sonicators
Posted 21 September 2004 - 02:08 PM
Any help would be most appreciated.
Posted 22 September 2004 - 09:29 AM
Thanks. The one you mention has 500 watts capacity. Do you think that is necessary? There are others that function at 100 watts max. I'm trying to find the best item at the lowest cost.
Again, Thanks very much for the info.
Posted 15 December 2004 - 06:24 AM
Misonix makes a 100 watt sonicator that can be used for DNA shearing. Here is the product page:
However, the advantage of using a more powerful unit (Misonix also makes a 600 watt model) is that usually, those units are programmable. Reproducibility is very important when doing Chip Assays and the lower units are typically controlled manually.
Normal ultrasonic bath sonicators are not strong enough to fragment DNA. However, cuphorns can be used to sonicated sealed vessels in a similar way as a bath, expect that the sonication is much stronger. The following is a link to the product page for cup horns. DNA shearing has been done in cuphorns before.