I am about to begin some ChIP assays on muscle tissue and hope to use this assay relatively frequently over the next few years. Thus, I was wondering what type of sonicator do most of you use for such assays. It seems that the standard is the Branson 250? probe sonicator. What about a bath sonicator? Have any of you had much success with the bath style sonicator? I have seen a few published protocols using bath sonicators, but most use probe sonicators for fragmenting the DNA.
Any help would be most appreciated.
tng
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#2
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Posted 21 September 2004 - 03:17 PM
We use a Fisher 500 Sonic Dismembrator which is well situated for ChIP sonication.
Here is the link to the product page
https://www1.fishers...41561&details=Y
Here is the link to the product page
https://www1.fishers...41561&details=Y
Edited by bioforum, 21 September 2004 - 10:24 PM.
#3
thenewguy
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Posted 22 September 2004 - 09:29 AM
Twister,
Thanks. The one you mention has 500 watts capacity. Do you think that is necessary? There are others that function at 100 watts max. I'm trying to find the best item at the lowest cost.
Again, Thanks very much for the info.
tng
Thanks. The one you mention has 500 watts capacity. Do you think that is necessary? There are others that function at 100 watts max. I'm trying to find the best item at the lowest cost.
Again, Thanks very much for the info.
tng
#4
sonix82
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Posted 15 December 2004 - 06:24 AM
Hi,
Misonix makes a 100 watt sonicator that can be used for DNA shearing. Here is the product page:
http://www.misonix.c...4&div=11&cat=21
However, the advantage of using a more powerful unit (Misonix also makes a 600 watt model) is that usually, those units are programmable. Reproducibility is very important when doing Chip Assays and the lower units are typically controlled manually.
Normal ultrasonic bath sonicators are not strong enough to fragment DNA. However, cuphorns can be used to sonicated sealed vessels in a similar way as a bath, expect that the sonication is much stronger. The following is a link to the product page for cup horns. DNA shearing has been done in cuphorns before.
http://www.misonix.c...6&div=11&cat=21
Misonix makes a 100 watt sonicator that can be used for DNA shearing. Here is the product page:
http://www.misonix.c...4&div=11&cat=21
However, the advantage of using a more powerful unit (Misonix also makes a 600 watt model) is that usually, those units are programmable. Reproducibility is very important when doing Chip Assays and the lower units are typically controlled manually.
Normal ultrasonic bath sonicators are not strong enough to fragment DNA. However, cuphorns can be used to sonicated sealed vessels in a similar way as a bath, expect that the sonication is much stronger. The following is a link to the product page for cup horns. DNA shearing has been done in cuphorns before.
http://www.misonix.c...6&div=11&cat=21
