From: Robbie, firstname.lastname@example.org
Date: 24 Jun 1999
Analogously to Southerns, I am trying to hybridize two strands of ssDNA to each otherin a TEST TUBE. I know that these strands have 25 bp homology, and one strand (thenon-probe strand--I call that the target strand) is 50 bp long. My question is what do yourecommend as the buffer conditions? I am unsure of the amounts of SSC/SDS, as this isdifferent from a strict Southern on nitro- cellulose. Also, do you have any suggestions onhow I can do a "wash" in this test-tube quasi-Southern method?
Thank you for any help you can provide!