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[anonymous]

Replacing the tyrosine with a glutamic acid residue is not necessarily a good idea.  Many phosphorylated tyrosines serve as docking sites for SH2 or PTB domains.  Glutamic acid residues can not substitute for phosphorylated tyrosines in such interactions. The mentioned approach is more likely to be successful for serine or threonine  residues.

From: Robbie, rshahman@aol.com
Category: DNA-Related
Date: 24 Jun 1999
Time: 13:58:06

Comments

Analogously to Southerns, I am trying to hybridize two strands of ssDNA to each otherin a TEST TUBE. I know that these strands have 25 bp homology, and one strand (thenon-probe strand--I call that the target strand) is 50 bp long. My question is what do yourecommend as the buffer conditions? I am unsure of the amounts of SSC/SDS, as this isdifferent from a strict Southern on nitro- cellulose. Also, do you have any suggestions onhow I can do a "wash" in this test-tube quasi-Southern method?

Thank you for any help you can provide!

Ravi