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Standard HCL protein hydrolysis technique without vacuum

hydrolysis vacuum

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#1 mariiamaak



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Posted 31 March 2020 - 05:31 PM

Hello everyone,


I am trying to follow the standard protocol for digesting proteins to amino acids. The hydrolysis needs to be done under vacuum. As I don't have an access to the vacuum setup, can I just purge my tubes with nitrogen and incubate them as recommended for 24 h at 110C?


Here is the method.


Thanks for responses!


#2 mdfenko


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Posted 01 April 2020 - 05:46 PM

According to the procedure to which you link, acid hydrolysis can be performed by reflux or in a sealed tube either in vacuum or nitrogen. It’s been a long time since i performed the procedure and don’t remember but i think i did it under vacuum (i only did it 2 or 3 times).

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