Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Standard HCL protein hydrolysis technique without vacuum

hydrolysis vacuum

  • Please log in to reply
1 reply to this topic

#1 mariiamaak

mariiamaak

    member

  • Members
  • Pip
  • 2 posts
1
Neutral

Posted 31 March 2020 - 05:31 PM

Hello everyone,

 

I am trying to follow the standard protocol for digesting proteins to amino acids. The hydrolysis needs to be done under vacuum. As I don't have an access to the vacuum setup, can I just purge my tubes with nitrogen and incubate them as recommended for 24 h at 110C?

 

Here is the method.

 

Thanks for responses!

Mariia



#2 mdfenko

mdfenko

    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,355 posts
214
Excellent

Posted 01 April 2020 - 05:46 PM

According to the procedure to which you link, acid hydrolysis can be performed by reflux or in a sealed tube either in vacuum or nitrogen. It’s been a long time since i performed the procedure and don’t remember but i think i did it under vacuum (i only did it 2 or 3 times).


talent does what it can
genius does what it must
i used to do what i got paid to do





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.