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Alkaline lysis for pBR322


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#1 vijay

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Posted 18 September 2004 - 11:02 AM

Hi all
I am always getting chromosomal DNA when isolating plasmid by alkaline lysis. Can anybody help me to get rid of this?
Thanks
Vijay

#2 hubeimm

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Posted 21 September 2004 - 02:20 PM

Hi, Vijay,
I think you maybe use too long time for lysising your cells and you didnt do it gently. You have to follow the protocol. If you let it stand only 3-4 min after you add lysis solution and invert gently (don't use Vortex). the outcome maybe improved.




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