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Degradation of GST fusion proteins


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#1 biotech

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Posted 17 September 2004 - 11:04 AM

I clone GST fusion proteins with pGEX 4T-3 vector, I check for inserts in minipreps (OK), grow bacteria, induce them, and run lysates on SDS-PAGE. What happens is that the expressed proteins are of GST size, probably degraded. Has anyone got that kind of results before? I checked DNA, it's OK, no stop codons at the ligation site.




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