I'm new to this forum so I hope I'm doing this right!
I am involved in a post-doctoral project looking at microbial diversity on feathers. We are currently attempting to amplify bacterial DNA directly from the feathers (no culturing) using conserved primers and are having some difficulties. We are essentially getting very little or no product at all. Lately, the PCR products have been smeared. Would anyone have any helpful comments or any suggestions? We are starting to wonder whether there is simply just very little bacteria on the feathers.
Thank you in advance.
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