1 reply to this topic

[bunster]

Hi there,

Does anyone have a reliable and simple method for culturing mouse splenocytes?  I need to stimulate them with mitogen and then I want to look at cytokine production.  Any culture medium that works really well?

Also, I am looking for a good protocol for protein extraction, either a commercially available kit or a tried and tested method from someone's lab.  I used to do Wblots and the protocol used a protease inhibitor set that was in a pellet that could be dissolved into the lysis buffer each day.  Can anyone help with this issue?  Thanks very much

Bunster

[jadefalcon]

hi

maybe a look at the "mouse splenocyte prep." topic in the pinned section could give you a clue.

the medium I use for mouse splenocytes is RPMI 1640, 10%FCS, 20mM Hepes, if neccesary 1% Pen/Strep.

mike