How can I purify these nonautoclavables? And do I need to?
Posted 16 September 2004 - 01:11 PM
I can autoclave or filter sterilize everything else in the procedure. Ordering new of any of these can be done too if neccessary.
Posted 16 September 2004 - 08:37 PM
You could try to re-purify the enzymes using an affinity column, though this may be more trouble than it is worth.
Best of luck
Posted 06 October 2004 - 03:40 PM
I would assume that your other components are RNase and DNase free.