Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

RT-PCR


  • Please log in to reply
2 replies to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 11 July 2001 - 09:00 PM

Don't know about lowest levels, but adding acetylated bsa is supposed to help (life technologies focus journal)i have not tried it though

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 11 July 2001 - 09:00 PM

Hi.How to increase the sensitivity of one step RT-PCR?What is the lowerest level it can detect?Thanks

#3 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 11 July 2001 - 09:00 PM

Pick a kit that was designed for sensitivity. We saw some advertisement for a kit from Sigma andthey were right. The kit did really well with low abundance RNA. But the best way is to simply try a kitand maybe it works.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.