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Unsuccessfull DNA extraction

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#1 confstud



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Posted 15 September 2004 - 03:15 PM

I have extracted DNA from Oenococcus oeni and When I've measured O.D. I got realyy good values but when I made it run in an agarose gel I realized I didn't have DNA (except for one sample) ¿What am I measuring with my 260 O.D.? :unsure:

#2 phage434



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Posted 15 September 2004 - 06:50 PM

Genomic DNA will typically not run on a gel (it's far too large), but rather show as a bright regions in or near the wells where it is loaded. That might be your problem.

Another possibility is that you have used a phenol chloroform extraction, and are measuring contaminating phenol at 260 nm.

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