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ROX concentration


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#1 Loubna

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Posted 15 September 2004 - 01:05 PM

Hi,
Does anybody know what the ROX dye concentration in the universal PCR mastermix from ABI?
I can't find it in their website...
Thank you for your help
L

#2 tfitzwater

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Posted 16 September 2004 - 10:01 AM

The 1X TaqMan Buffer A formulation is available from ABI, but basically it is ABI PCR buffer plus ROX at 60 nM at 1X Concentration. Synthegen ROX is available as 5-(and 6-)carboxy-X-rhodamine or 6-)carboxy-X-rhodamine. At 60 nM, the signal is somewhat lower than ABI's signal, but this does not seem to be a problem. ROX is also available from Pierce Chemical or Invitrogen.




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