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I would greatly appreciate assistance in running mouse platelets on the flow cytometer. Although I am able to identify my platelets by CD61 staining, I am still having difficulty with identifying platelets and platelet derived microparticles. Does anyone do this kind of work, and if so, would they be able to assist me in setting my parameters? I found that the platelets and microparticle populations are best identified when both SSC and FSC are set on log scale and the gain is set on e01. Even with these settings, I am picking up a great deal of non-specific events even when I try to set the flow using purified washed resting platelets. Thanks. 
Edited by immune platelet, 12 October 2004 - 10:04 AM.
