How little competent cell do you use for transformation

Started by postdoc, Sep 14 2004 08:06 AM

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#1 postdoc

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Posted 14 September 2004 - 08:06 AM

Usually companies selling competent cells encourage you to use as much as possible and 100 ul is suggested. Because they are not cheap, I have used 50 ul per transformation. How much can I further scale down?

Thanks.

#2 turtle

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Posted 14 September 2004 - 08:51 AM

I routinely use 25 ul. Note that i am cloning single PCR bands for sequencing, not building a library! I sometimes have low efficiency problems, but I don't think this is related to using only 25 ul. I routinely get several hundred colonies (I plate the entire transformation reaction).
Turtle