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[Benwilliams]

Does anyone have any suggestions for correcting differential amplification in PCRs on VNTR markers?

My pcr is for a 48bp repeat ranging from 300bp for the 2 repeat to 540bp for the 7 repeat. Longer alleles give very low yields in heterozygous samples, often dropping out completely

I am using template collected via buccal swabs and have tried altering extension times and buffer concentrations as well as DMSO and deaza dNTPs.

Any suggestions?

Many thanks

Ben