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ISSR - electrophoresis


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#1 wapa

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Posted 14 September 2004 - 12:49 AM

Hi everybody

I would like to use ISSR as molecular markers. I have found two methods to visualise PCR products : polyacrylamide-silver staining gel and Agarose gel. Can you tell me what is the best method, and the gel concentration usualy used?

thank you in advance

#2 Nina

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Posted 14 September 2004 - 07:20 AM

When i want to analyze if i have PCR product,
I use 2% agarose gel stained with EtBr (ethidiumbromide) ~5 Ál EtBr to a 50 ml agarose gel.

But the concentration of the gel is dependent on wich size our fragment have.

I use 2% to a fragment size of 150bp.

Good luck!

/Nina

#3 wapa

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Posted 15 September 2004 - 06:48 AM

thank for you for your answer.
good luck too




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