Hi everybody
I would like to use ISSR as molecular markers. I have found two methods to visualise PCR products : polyacrylamide-silver staining gel and Agarose gel. Can you tell me what is the best method, and the gel concentration usualy used?
thank you in advance
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#2
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Posted 14 September 2004 - 07:20 AM
When i want to analyze if i have PCR product,
I use 2% agarose gel stained with EtBr (ethidiumbromide) ~5 µl EtBr to a 50 ml agarose gel.
But the concentration of the gel is dependent on wich size our fragment have.
I use 2% to a fragment size of 150bp.
Good luck!
/Nina
I use 2% agarose gel stained with EtBr (ethidiumbromide) ~5 µl EtBr to a 50 ml agarose gel.
But the concentration of the gel is dependent on wich size our fragment have.
I use 2% to a fragment size of 150bp.
Good luck!
/Nina
#3
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Posted 15 September 2004 - 06:48 AM
thank for you for your answer.
good luck too
good luck too
