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ELISA normalisation


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#1 kant0008

kant0008

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Posted 13 September 2004 - 11:12 PM

Hey all,
just wondering, when you do ELISA do you dilute your samples to a uniform total protein concentration and then do the assay, or is it ok to use eg. a constant volume of sample and then work out the concentration of protein of interest later, based on a Bradford?
Thanks!




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