About R28 cells culture
Posted 10 September 2004 - 11:41 PM
Have anyone met the same problem?
Posted 12 September 2004 - 04:56 PM
how much G418 are you using? They could have some "natural" resistance, depends on what's your dose?
Also, if they have been sitting in culture side by side with transfected lines- unlikely but possible (happened in out lab) cross-contamination can occur. Check for plasmid by PCR?
Also, someone earlier had the same problem with cells that they got from someone else- maybe you don't have all the info?
Hope this helps
Posted 13 September 2004 - 06:58 AM
So the only reason perhaps is that the cell is a gift from other labs.
Posted 06 March 2012 - 09:57 AM
Establishment of an E1A-immortalized retinal cell culture.
In Vitro Cell Dev Biol Anim. 1996 Feb;32(2):66-8.