Thank you pcrman!
I went onto this website but got rather confused as I dont have much experience of analyzing genes. I know there are 2 promoters in my gene of interest PPARGC1A. I would like to do a sequence alignment of both promoters comparing human to other species. Could you possibly give me an example of how to find the TSS and the upstream section using the example of PPARGC1A?
I have attached a screenshot of what came up but I cant work out where the TSS is or where the promoter is located? How much further upstream do I need to go to locate the 2 promoters in my gene and how do I find these promoter sequences?
If you can give me a step by step for PPARGC1A in human, I should be able to get the other species myself following your instructions.
After alignment, the next step is to find a specific motif in the two promoters that a specific transcription factor binds to which is: GTCACGTG.
Really appreciate the help.