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Unusual Issues with Confocal of Agro-infiltrated Tobacco

Confocal

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#1 AAV

AAV

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Posted 04 March 2020 - 10:37 AM

I cloned a couple of proteins with GUS into pMDC vectors to confirm localization in Tobacco and agroinfiltrated benthamiana leaves with my construct in GV3101 along with p19 and analyzed the leaf discs at 3dpi in confocal microscope. In some of the samples I see blobs in addition to GFP signals in the leaf samples. I repeated this experiment a couple of times but it doesn’t seem to go. This is the first time I have encountered this problem although I have done similar experiments earlier without any issues. Has anyone encountered this kind of a problem? Any suggestions, tip or info is highly appreciated! Thanks!!


Edited by AAV, 04 March 2020 - 10:40 AM.






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