3 replies to this topic

[kindmike]

Hi all,
we are building a new molecular biology lab, mainly focus on gene expression, localize the expression of genes and Immunohistochemistry. Could somebody give us some suggestion on when building such kind of lab, what kind of chemical regular solution, kits and instruments do we need. For budget purposes, If someone can provide detail prices and names, it will be highly appreciated. Thanks.

[BryanW]

Molecular Biology - gene expression
Reagent required:
(1) nucleic acid purification kits - Qiagen, Epoch Biolabs (among best quality with Epoch the most cost-effective)
(2) PCR/cloning - real time quantitative RT-PCR (Taqman/lifecycler- Roche);cloning/expression vector - Invitrogen (best quality and cost-effective)
(3) protein expression - His-tag/Ni-column - Qiagen, Invitrogen
(4) In situ hybridization - anti-DIG - Roche
(5) Immunocytochemistry/immunofluoresencce/Western - antibodies - BD Bioscience, Labvision, Biolegend
(6) cell culture - media - Invitrogen/GIBCO (best quality and cost-effective)
(7) cell transfection - GenCarrier-1 - Epoch Biolabs (best quality and cost-effective)
(8) cytokines/growth factors - R&D Systems, PeproTech, Epoch Biolabs (among the best quality)
(9) microarray chip - premade or custom chip - Affimetrix or common facility of major University

Anything else?

[jadefalcon]

-incubation closet
-autoclav
-MilliQ
-centrifuges

mike

[kant0008]

-microscope
-cryostat

For budget purposes you can get at least some ideas from company websites, such as Promega, Roche, Invitrogen, Qiagen, etc. Otherwise Biocompare.com is not too bad, though some prices are not up to date, but it gives you a range of manufacturers for what you need