Edited by biogirl1230, 14 February 2020 - 01:09 PM.
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probe pool calculation
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Posted 14 February 2020 - 01:07 PM
I have a probe pool at 1000 probes in a single pool. The pool concentration is 20nM. We use 5ul of this into our ligation reaction. I calculated this out as 100fmol/reaction (20nM*5ul), then 0.1 fmol/probes (100fmol/1000probes).
We ordered some new probes to spike into the 20nM pool. We ordered them as 10 probes in lyophiized (dried) form. We can resuspend dried tube how we want, then spike in some volume of it into the original 20nM probe pool. We want the final molarity to stay as above, 0.1fmol/probe. What is the easiest way to do this and make sure each probe (old and new) are at the same molarity in the end? Thanks in advance.