I am a PhD student. I am planning a small study that will use the immunohistochemistry technique. But I don't know how to choose the primary antibody to use.
I took paraffin sections using rat liver samples. I search the internet for companies primer antibody that I will use. For example; abcam, santa cruz...
What should I look out for in polyclonal, monoclonal, conjugated, unconjugated, host species,or description sections?
I have anti rabbit as secondary antibody. Do I have any importance for this primary antibody?
In short, what are the things to consider when choosing antibodies for immunohistochemistry?