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What concentration?

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#1 tadeo333



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Posted 10 January 2020 - 07:23 AM

Hi there  


And how much should I add 

I have a stock solution of Leupeptin of 2mg/ml.
I want to add 200 uM to my cells.
At the end of the day, I don't know what concentration and how much I need to get 200 uM of Leupeptin.
Here is the information regarding Leupeptin


Can anyone help out?
Sorry, I am not exactly a math genius right now.

Would greatly appreciate it!

Thank you greatly

#2 mdfenko


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Posted 10 January 2020 - 08:34 PM

What salt do you have (i can’t access the link)? Find the formula weight of the leupeptin salt you have, divide 2mg/ml by the fw (that gives you the molar concentration of the stock, remember that 2mg/ml=2gm/l). Then figure the dilution of that to 200 uM (c1v1=c2v2).

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