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Antigen Retrieval

histology antigen retrieval fixation PFA

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#1 SAT142



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Posted 02 January 2020 - 08:18 AM

I am fixing rat TMJ tissues in PFA, deossifying in immunocal, and then preparing for immunostaining. However, when PFA is used, no signal appears, so we have been testing different methods of antigen retrieval. No method has been successful, and we are not sure what is causing the issue. We have fixed tissues in PFA for a day and also a few hours to see if the fixation time is affecting the stain. Our antigen retrieval method is 20 minutes in sodium citrate at 95 degrees in a water bath. Please let me know if you have had this issue or have any advice on how to fix it. 

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