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viral RNA extraction kit

Started by SF_HK, Dec 29 2019 11:21 PM

PCR DNA qPCR

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#1 SF_HK

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Posted 29 December 2019 - 11:21 PM

Hi,

I was extracting DNA from a kit to run Taqman qPCR for genotyping (SNP) detection. I just realised that the kit I used is for for viral RNA extraction and does not designed to separate viral RNA from DNA. So this implies my eulant has RNA and DNA contamination? I was using human plasma for extraction.

Does presence of RNA could be affecting the qPCR run, even though the taqman probes are specific to DNA?

Edited by SF_HK, 29 December 2019 - 11:25 PM.

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