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nested PCR

amplicon length

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#1 SF_HK



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Posted 08 December 2019 - 11:52 PM



I plan to use nested PCR on urine DNA.  I have never performed this type of PCR and would appreciate any advice.


My target size is about 60-80bp.


My question is how long should my first round PCR amplicon be if my second round PCR product  size is between 60-80bp?


Thank you



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