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cell-free DNA from urine


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#1 SF_HK

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Posted 08 December 2019 - 05:54 PM

Hi,

 

What is the best method to separate ow molecular weight DNA from high molecular weight DNA from urine. Would anyone have a protocol to cell-free DNA from urine. eve tried 2 kits and none of them give a good yield. 



#2 samgreenberg

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Posted 30 December 2019 - 02:11 PM

I have had good results with Qiagen kits (though only from serum and not from urine). Have you tried this kit: https://www.qiagen.c...ringinformation ?

 

There are a couple ways to separate DNA by molecular weight. Gel purification and magnetic beads are probably the best options. I would recommend magnetic beads, since the mass of cfDNA recovered is often so low you will never see it on a gel. However, I would question whether it is really necessary to separate cfDNA by molecular weight for most experiments.  






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