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spike-in control


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#1 SF_HK

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Posted 08 November 2019 - 06:06 PM

Hello,

 

Im new to microRNA. As all qPCR reactions have to be normalised, can someone help  me understand what is spike-in control for miRNA normalization and how it work?

 

When do you we use endogenous miRNA-xx and when do we use spike-in control for quantification, doe sit depend on sample type?

 

thank you

 



#2 bob1

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Posted 09 November 2019 - 12:53 AM

A spike-in control is when you add a known amount of a known target to a reaction. You use this when you need to check that your reactions are working as expected and your data is the result of correct amplification of your target genes. You wouldn't normally use it as a standard for quantitation other than you can use it to create a standard curve for copy-number determination.




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