Troubleshooting sds-page for high mw protein
Posted 10 September 2004 - 01:12 AM
The longer the boil, the greater is the smear at the top of my membrane (after blotting). Anyone has any idea of what the problem could be? I used the Mem-Per kit from Pierce to isolate the proteins and the solution B for this kit to dilute my sample prior to boiling.
Thank you for your suggestions in advance.
Posted 23 September 2004 - 11:36 AM
Posted 18 October 2004 - 01:23 AM