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Things you should consider before pipetting

PIPETTING ACCURACY PIPETTING TECHNIQUE

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#1 SCIENCE GEEK

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Posted 09 October 2019 - 05:26 AM

Before Use                                                                                          
  • Clean your pipettes every day before use.
  • Use tip that is well-fitted so that the air does not escape while drawing up and dispensing liquid.
  • Learn to pipette by practising a few times before doing the actual experiment.
  • Pre-wet the tip before the experiment. This is because when you dispense the liquid from a pipette, it leaves a layer of liquid on the tip, resulting in slightly lower dispensed volume. If you pre-wet the tip, this will not happen. In order to follow the pre-wet process, draw the liquid into the pipette and then dispense it back to the vessel from which it was taken. You will see the coating on the tip. Next time you repeat the action, the liquid coating will not be left.





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