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Possible to sort cells from formalin?


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#1 Daisy999

Daisy999

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Posted 15 September 2019 - 01:18 AM

Hi,

ufabet

I don't think it's possible to sort from formalin fixed tissues. But then I haven't tried smile.png I usually ask to get the biopsies fresh, that is unfixed direct from the patient, into complete medium on ice. Then use first manual mincing (rotary cutter!) and Dispase/Collagenase for 2hrs at 37C.

 

Filter, wash, stain, sort. BW, rsm Hi, I don't think it's possible to sort from formalin fixed tissues. But then I haven't tried smile.png I usually ask to get the biopsies fresh, that is unfixed direct from the patient, into complete medium on ice.

 

Then use first manual mincing (rotary cutter!) and Dispase/Collagenase for 2hrs at 37C. Filter, wash, stain, sort. BW, rsm 

I don't think it's possible to sort from formalin fixed tissues. But then I haven't tried smile.png

I usually ask to get the biopsies fresh, that is unfixed direct from the patient, into complete medium on ice. Then use first manual mincing (rotary cutter!) and Dispase/Collagenase for 2hrs at 37C. Filter, wash, stain, sort.


Edited by Daisy999, 15 September 2019 - 01:19 AM.





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