Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

HRM analysis of PCR product from non-real-time instrument

  • Please log in to reply
No replies to this topic

#1 Paja



  • Active Members
  • PipPipPipPipPip
  • 33 posts

Posted 17 June 2019 - 03:31 AM


I would like to perform HRM analysis (on LC480 machine). I have already run PCR with buffer containing intercalating dye (dedicated for HRM analysis) on the regular PCR instrument. Can I use these PCR products, transfer them to LC480 and perform only HRM? If yes, do the PCR prosucts need to be kept in freezer between PCR and HRM (time between the two procedures is approx. 7 days)? How shall I set the melting analysis temperature /time profile on LC480?

Thank you very much for any comment and advice.


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.