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actinomycin D pretreatment

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#1 SF_HK



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Posted 26 May 2019 - 08:13 PM

My drug is increasing proliferation of gene of interest (GOI).  I would like to demonstrate the effect of this drug at the transcriptional level.     


I plan to:


1. pre-treat my cells with Actinomycin D alone at various time points

2. add my drug after pretreatment with actinomycin.  


My question is for part 2, after pre-treatment with actinomycin, do I still add actinomycin with the drug or do I  remove actinomycin D and just add my drug to the cells?


Ive never worked with actinomycin D before


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