Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

actinomycin D pretreatment


  • Please log in to reply
No replies to this topic

#1 SF_HK

SF_HK

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 238 posts
5
Neutral

Posted 26 May 2019 - 08:13 PM

My drug is increasing proliferation of gene of interest (GOI).  I would like to demonstrate the effect of this drug at the transcriptional level.     

 

I plan to:

 

1. pre-treat my cells with Actinomycin D alone at various time points

2. add my drug after pretreatment with actinomycin.  

 

My question is for part 2, after pre-treatment with actinomycin, do I still add actinomycin with the drug or do I  remove actinomycin D and just add my drug to the cells?

 

Ive never worked with actinomycin D before

Thanks






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.