My drug is increasing proliferation of gene of interest (GOI). I would like to demonstrate the effect of this drug at the transcriptional level.
I plan to:
1. pre-treat my cells with Actinomycin D alone at various time points
2. add my drug after pretreatment with actinomycin.
My question is for part 2, after pre-treatment with actinomycin, do I still add actinomycin with the drug or do I remove actinomycin D and just add my drug to the cells?
Ive never worked with actinomycin D before