I am trying to do buffer exchange by simple dilution. I have 200ul of protein in Buffer A, 20mM and 50mM NaCl. I want to dilute it with buffer B which is 25mM and NaCl 40mM. I want the buffers to exchange but the salt concentration to be 40mM-50mM.How much buffer would I add to the 200uL of protein? Will making it upto 2ml be enough?
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Dilution buffer
Started by Minmax82, May 01 2019 04:53 AM
1 reply to this topic