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Dilution buffer

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#1 Minmax82



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Posted 01 May 2019 - 04:53 AM

I am trying to do buffer exchange by simple dilution. I have 200ul of protein in Buffer A, 20mM and 50mM NaCl. I want to dilute it with buffer B which is 25mM and NaCl 40mM. I want the buffers to exchange but the salt concentration to be 40mM-50mM.How much buffer would I add to the 200uL of protein? Will making it upto 2ml be enough?

#2 bob1


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Posted 01 May 2019 - 06:14 AM

Because the salt concentrations are so similar  - 40 mM and 50 mM, mixing them at a 1:1 ratio will give you a final concentration of 45 mM NaCl. This would mean adding 200 ul of buffer B mix to the 200 ul of protein already dissolved in buffer A.

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