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Calcium Imaging Calibration

Calcium Imaging Ionomycin EGTA Neuroblastoma cells

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#1 CristianaCris

CristianaCris

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Posted 29 April 2019 - 11:58 AM

Hi everyone,

 

I want to do Calcium Imaging (neuroblastoma cells) and to get the minimum response by using Ionomycin + EGTA and maximum response by using Ionomycyn + CaCl2.

My question is: Which would be the proper concentrations of Ca and EGTA in order to get a transient response curve within 60 seconds? The response curve that I obtain is quite stable for Ca and for EGTA it takes around 300 sec.

 

And which would be the parameters to take into accound when optimizing the experiment?

 

Thank you.

 

 


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Also tagged with one or more of these keywords: Calcium Imaging, Ionomycin, EGTA, Neuroblastoma cells

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