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Calcium Imaging Calibration

Calcium Imaging Ionomycin EGTA Neuroblastoma cells

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#1 CristianaCris

CristianaCris

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Posted 29 April 2019 - 11:58 AM

Hi everyone,

 

I want to do Calcium Imaging (neuroblastoma cells) and to get the minimum response by using Ionomycin + EGTA and maximum response by using Ionomycyn + CaCl2.

My question is: Which would be the proper concentrations of Ca and EGTA in order to get a transient response curve within 60 seconds? The response curve that I obtain is quite stable for Ca and for EGTA it takes around 300 sec.

 

And which would be the parameters to take into accound when optimizing the experiment?

 

Thank you.

 

 







Also tagged with one or more of these keywords: Calcium Imaging, Ionomycin, EGTA, Neuroblastoma cells

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